Infl uence Escherichia coli D 21 g Surface Chemistry and Transport in Saturated Sand
نویسندگان
چکیده
2108 Th e eff ect of cell preparation methods on the surface chemistry and retention of Escherichia coli D21 g was investigated over a range of ionic strength conditions. Th e cell preparation methods that were considered included fi ltration and centrifugation (at various speeds and for diff erent durations). For a given ionic strength condition, it was found that cells prepared by fi ltration were more negatively charged and hydrophobic than cells prepared by centrifugation. Increasing the centrifugation speed (force imposed) or duration produced cells with a higher zeta potential (less negative) and a lower hydrophobicity. Column transport experiments for E. coli D21 g were also conducted with ultra pure quartz sand and the same solution chemistries. Th e fi rst-order retention rate coeffi cient for E. coli D21 g increased with increasing speed and duration of centrifugation, and was lowest in the case of fi ltered cells. Moreover, the infl uence of cell preparation method was more pronounced in lower ionic strength solutions. Cell Preparation Methods Infl uence Escherichia coli D21g Surface Chemistry and Transport in Saturated Sand
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